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High specificity detection of Pb

Ruozhen YU,Zheng FANG,Wei MENG,Zhenguang YAN,Lina DU,Hong WANG,Zhengtao LIU

Frontiers of Environmental Science & Engineering 2014, Volume 8, Issue 5,   Pages 729-736 doi: 10.1007/s11783-013-0611-3

Abstract: The conjugates of keyhole limpet hemocyanin (KLH) and -SCN-Bz-DTPA-Pb were used as immunogen, whereasthe conjugates of bovine serum albumin (BSA) and -NH -Bn-DTPA-Pb were used as coating antigen.Polyclonal antibodies specific to DTPA-Pb chelates were obtained from rabbits.In the mixing microplate, DTPA and Pb ions formed chelates and combined with specific antibodies.The reaction microplate was coated by the conjugates of BSA and -NH -DTPA-Pb, which competed for the

Keywords： <i>pi>-SCN-Bz-DTPA     <i>pi>-NH2-Bn-DTPA     lead ion     polyclonal antibody    

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Differences in Immunoglobulin G Glycosylation Between Influenza and COVID-19 Patients Article

Marina Kljaković-Gašpić Batinjan, Tea Petrović, Frano Vučković, Irzal Hadžibegović, Barbara Radovani,Ivana Jurin, Lovorka Đerek, Eva Huljev, Alemka Markotić, Ivica Lukšić, Irena Trbojević-Akmačić, Gordan

Engineering 2023, Volume 26, Issue 7,   Pages 54-62 doi: 10.1016/j.eng.2022.08.007

Abstract:

The essential role of immunoglobulin G (IgG) in immune system regulation and combatting infectious diseases cannot be fully recognized without an understanding of the changes in its N-glycans attached to the asparagine 297 of the Fc domain that occur under such circumstances. These glycans impact the antibody stability, half-life, secretion, immunogenicity, and effector functions. Therefore, in this study, we analyzed and compared the total IgG glycome—at the level of individual glycan structures and derived glycosylation traits (sialylation, galactosylation, fucosylation, and bisecting N-acetylglucosamine (GlcNAc))—of 64 patients with influenza, 77 patients with coronavirus disease 2019 (COVID-19), and 56 healthy controls. Our study revealed a significant decrease in IgG galactosylation, sialylation, and bisecting GlcNAc (where the latter shows the most significant decrease) in deceased COVID-19 patients, whereas IgG fucosylation was increased. On the other hand, IgG galactosylation remained stable in influenza patients and COVID-19 survivors. IgG glycosylation in influenza patients was more time-dependent: In the first seven days of the disease, sialylation increased and fucosylation and bisecting GlcNAc decreased; in the next 21 days, sialylation decreased and fucosylation increased (while bisecting GlcNAc remained stable). The similarity of IgG glycosylation changes in COVID-19 survivors and influenza patients may be the consequence of an adequate immune response to enveloped viruses, while the observed changes in deceased COVID-19 patients may indicate its deviation.

Keywords： Influenza     COVID-19     Viral infection     Glycosylation     Immunoglobulin G     Pneumonia    

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Review of 4Pi Fluorescence Nanoscopy Review

Xiang Hao, Yiming Li, Shuang Fu, Yanghui Li, Yingke Xu, Cuifang Kuang, Xu Liu

Engineering 2022, Volume 11, Issue 4,   Pages 146-153 doi: 10.1016/j.eng.2020.07.028

Abstract: An important milestone in this pursuit is the coherent utilization of two opposing objectives (4Pi geometryHerein, we review the recent progress in 4Pi nanoscopy, which provides a 3D, non-invasive, diffraction-unlimitedThis review includes both the targeted and stochastic switching modalities of 4Pi nanoscopy.The schematics, principles, applications, and future potential of 4Pi nanoscopy are discussed in detail

Keywords： Fluorescence nanoscopy     Super-resolution imaging     4Pi nanoscopy     IsoSTED     4Pi-SMLM    

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RGS16 regulated by let-7c-5p promotes glioma progression by activating PI3K-AKT pathway

Frontiers of Medicine 2023, Volume 17, Issue 1,   Pages 143-155 doi: 10.1007/s11684-022-0929-y

Abstract: Ectopic deletion of RGS16 significantly suppressed glioma cell proliferation and migration both <i>invitroi> and <i>in vivoi>.

Keywords： RGS16     let-7c-5p     glioma     proliferation     migration    

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Xiao Ke Qing improves glycometabolism and ameliorates insulin resistance by regulating the PI3K/Akt pathway

Xiaoqing Li, Xinxin Li, Genbei Wang, Yan Xu, Yuanyuan Wang, Ruijia Hao, Xiaohui Ma

Frontiers of Medicine 2018, Volume 12, Issue 6,   Pages 688-696 doi: 10.1007/s11684-018-0662-8

Abstract: Moreover, XKQ can ameliorate insulin resistance and upregulate the expression of IRS-1, PI3K (p85), p-AktXKQ is an effective drug for T2DM by ameliorating insulin resistance and regulating the PI3K/Akt signaling

Keywords： XKQ     type 2 diabetes mellitus     KKAy mice     PI3K/Akt pathway     diabetic liver disease    

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Ultraviolet-B induced expression of hypoxia-inducible factor 1α, transferrin receptor through EGFR/PI3K

LI Yanhua, BI Zhigang

Frontiers of Medicine 2007, Volume 1, Issue 1,   Pages 79-86 doi: 10.1007/s11684-007-0016-4

Abstract: The EGFR/PI3K/AKT signaling pathway was required for the induction of HIF-1α and TfR expression inducedPI3K inhibitors, LY294002 and wortmannin, inhibited HIF-1?We conclude that UVB induces TfR and HIF-1αexpressions via EGFR/PI3K/AKT/DEC1 signaling pathway.
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Micro － blind mesh thermal control study and simulation of nano-satellite

Yang Juan,Li Yunze,Wang Jun

Strategic Study of CAE 2008, Volume 10, Issue 7,   Pages 169-172

Abstract: dynamic characteristics model of the spherical nano-satellite was established.Based on the model,a fuzzy PI

Keywords： nano-satellite     micro-blind mesh     fuzzy PI controller     simulation    

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Enhanced photoelectrochemical water splitting with a donor-acceptor polyimide

Frontiers in Energy doi: 10.1007/s11708-023-0910-8

Abstract: Polyimide (PI) has emerged as a promising organic photocatalyst owing to its distinct advantages of highHowever, the synthesis of high-quality PI photoelectrode remains a challenge, and photoelectrochemical(PEC) water splitting for PI has been less studied.Herein, the synthesis of uniform PI photoelectrode films via a simple spin-coating method was reportednaphthalene, perylene) of electron acceptor on PEC performance were studied, where naphthalene-based PI

Keywords： polyimide (PI) film     photoelectrochemistry     band structure engineering     aromatic unit    

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Effects of mulching for water conservation on soil carbon, nitrogen and biological properties

Xiaomin PI, Tongxun ZHANG, Benhua SUN, Quanhong CUI, Yun GUO, Mingxia GAO, Hao FENG, David W.

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 2,   Pages 146-154 doi: 10.15302/J-FASE-2017136

Abstract: The effect of mulching with straw, white plastic film and black plastic film for improving soil water storage and on the soil properties was examined in a wheat-maize rotation system on Loess soil in northwestern China. All the mulches improved the water storage to some extent and led to warmer soils. However, the organic C and total N contents of the soils declined significantly under the mulches, particularly the plastic film mulches, and this could have long-term detrimental effects on soil quality and the sustainability of the some mulching practices.

Keywords： biomass     carbon     mulch     nitrogen     plastic films     soil     straw     water storage    

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Mutation analysis of KCNQ1, KCNH2, SCN5A, KCNE1 and KCNE2 genes in Chinese patients with long QT syndrome

DU Rong, TIAN Li, YUAN Guohui, LI Jin, REN Faxin, GUI Le, LI Wei, ZHANG Shouyan, KANG Cailian, YANG Junguo

Frontiers of Medicine 2007, Volume 1, Issue 3,   Pages 312-315 doi: 10.1007/s11684-007-0060-0

Abstract: Long QT syndrome (LQTS) is the prototype of the cardiac ion channelopathies, which cause syncope and sudden death. Inherited LQTS is represented by the autosomal dominant Romano-ward syndrome (RWS), which is not accompanied by congenital deafness, and the autosomal recessive Jervell and Lange-Nielsen syndrome (JLNS), which is accompanied by congenital deafness. The LQTS-causing mutations have been reported in patients and families from Europe, North America and Japan. Few genetic studies have been carried out in families with JLNS from China. This study investigates the molecular pathology in four families with LQTS (including a family with JLNS) in the Chinese population. Polymerase chain reaction and DNA sequencing were used to screen for , , , and mutation. A missense mutation G314S in an RWS family was identified, and a single nucleotide polymorphism (SNP) G643S was indentified in the of the JLNS family. In this JLNS family, another heterozygous novel mutation in exon 2a was found in of the patients. Our data provide useful information for the identification of polymorphisms and mutations related to LQTS and the Brugada Syndrome (BS) in Chinese populations.

Keywords： recessive Jervell     LQTS-causing     population     autosomal dominant     syndrome    

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A 3D porous WP2 nanosheets@carbon cloth flexible electrode for efficient electrocatalytic hydrogen evolution

Mingyu Pi, Xiaodeng Wang, Dingke Zhang, Shuxia Wang, Shijian Chen

Frontiers of Chemical Science and Engineering 2018, Volume 12, Issue 3,   Pages 425-432 doi: 10.1007/s11705-018-1726-7

Abstract:

Self-standing porous WP2 nanosheet arrays on carbon fiber cloth (WP2 NSs/CC) were synthesized and used as a 3D flexible hydrogen evolution electrode. Because of its 3D porous nanoarray structure, the WP2 NSs/CC exhibits a remarkable catalytic activity and a high stability. By using the experimental measurements and first-principle calculations, the underlying reasons for the excellent catalytic activity were further explored. Our work makes the present WP2 NSs as a promising electrocatalyst for hydrogen evolution and provides a way to design and fabricate efficient hydrogen evolution electrodes through 3D porous nano-arrays architecture.

Keywords： WP2     nanosheet arrays     hydrogen evolution electrocatalyst     flexible electrode    

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Performance of PI controller for control of active and reactive power in DFIG operating in a grid-connected

Azzouz TAMAARAT,Abdelhamid BENAKCHA

Frontiers in Energy 2014, Volume 8, Issue 3,   Pages 371-378 doi: 10.1007/s11708-014-0318-6

Abstract: the grid, based on vector control concept (with stator flux or voltage orientation) with classical PI

Keywords： wind power     doubly fed induction generator (DFIG)     vector control     active power     reactive power     maximum power point tracking (MPPT)    

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Precision control system of two-DOF stage with linear ultrasonic motor

ZHANG Hanlei, SHI Yunlai, ZHAO Chunsheng

Frontiers of Mechanical Engineering 2008, Volume 3, Issue 4,   Pages 421-425 doi: 10.1007/s11465-008-0063-9

Abstract: In this paper, a closed loop PI control system is designed to achieve high position accuracy during theThe PI control algorithm is used to increase the stability and accuracy of position control.

Keywords： settling     position accuracy     PI control     control algorithm     orthogonal    

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Development of a new method for RMR and Q classification method to optimize support system in tunneling

Asghar RAHMATI,Lohrasb FARAMARZI,Manouchehr SANEI

Frontiers of Structural and Civil Engineering 2014, Volume 8, Issue 4,   Pages 448-455 doi: 10.1007/s11709-014-0262-x

Abstract: Rock mass classification system is very suitable for various engineering design and stability analysis. classification method is confirmed by Japan Highway Public Corporation that this method can figure out either strength or deformability of rock mass, further appropriating the amount of rock bolts, thickness of shotcrete, and size of pitch of steel ribs just after the blasting procedure. Based on these advantages of method, in this study, according to data of five deep and long tunnels in Iran, two equations for estimating the value of method from and classification systems were developed. These equations as a new method were able to optimize the support system for and classification systems. From classification and its application in these case studies, it is pointed out that the method for the design of support systems in underground working is more reliable than the and classification systems.

Keywords： <i>JHi> classification     <i>Qi> and <i>RMRi> classification     new method    

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The Potential Role of Powdery Mildew-Resistance Gene <i>Pm40i> in Chinese Wheat-Breeding Programs inthe Post-<i>Pm21i> Era Perspective

Shengwen Tang,Yuting Hu,Shengfu Zhong,Peigao Luo

Engineering 2018, Volume 4, Issue 4,   Pages 500-506 doi: 10.1016/j.eng.2018.06.004

Abstract:

Powdery mildew, which is caused by Blumeria graminis f. sp. tritici (Bgt), is an important leaf disease that affects wheat yield. Powdery mildew-resistance (Pm) gene Pm21 was first transferred into wheat in the 1980s, by translocating the Heuchera villosa chromosome arm 6VS to the wheat chromosome arm 6AL (6VS·6AL). Recently, new Bgt isolates that are virulent to Pm21 have been identified in some wheat fields, indicating that wheat breeders should be aware of the risk of deploying Pm21, although pathological details regarding these virulent isolates still remain to be discovered. Pm40 was identified and mapped on the wheat chromosome arm 7BS from several wheat lines developed from the progenies of a wild cross between wheat and Thinopyrum intermedium. Pm40 offers a broad spectrum of resistance to Bgt, which suggests that it is likely to provide potentially durable resistance. Cytological methods did not detect any large alien chromosomal segment in the wheat lines carrying Pm40. Lines with Pm40 and promising agronomical traits have been released by several wheat-breeding programs in the past several years. Therefore, we believe that Pm40 will play a role in powdery mildew-resistance wheat breeding after Pm21 resistance is overcome by Bgt isolates. In addition, both Pm21 and Pm40 were derived from alien species, suggesting that the resistance genes derived from alien species are potentially more durable or effective than those identified from wheat.

Keywords： Wheat     Powdery mildew     <i>Pm21i>     <i>Pm40i>     Alien species     Native resistance    

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Title Author Date Type Operation

High specificity detection of Pb

Ruozhen YU,Zheng FANG,Wei MENG,Zhenguang YAN,Lina DU,Hong WANG,Zhengtao LIU

Journal Article

Differences in Immunoglobulin G Glycosylation Between Influenza and COVID-19 Patients

Marina Kljaković-Gašpić Batinjan, Tea Petrović, Frano Vučković, Irzal Hadžibegović, Barbara Radovani,Ivana Jurin, Lovorka Đerek, Eva Huljev, Alemka Markotić, Ivica Lukšić, Irena Trbojević-Akmačić, Gordan

Journal Article

Review of 4Pi Fluorescence Nanoscopy

Xiang Hao, Yiming Li, Shuang Fu, Yanghui Li, Yingke Xu, Cuifang Kuang, Xu Liu

Journal Article

RGS16 regulated by let-7c-5p promotes glioma progression by activating PI3K-AKT pathway

Journal Article

Xiao Ke Qing improves glycometabolism and ameliorates insulin resistance by regulating the PI3K/Akt pathway

Xiaoqing Li, Xinxin Li, Genbei Wang, Yan Xu, Yuanyuan Wang, Ruijia Hao, Xiaohui Ma

Journal Article

Ultraviolet-B induced expression of hypoxia-inducible factor 1α, transferrin receptor through EGFR/PI3K

LI Yanhua, BI Zhigang

Journal Article

Micro － blind mesh thermal control study and simulation of nano-satellite

Yang Juan,Li Yunze,Wang Jun

Journal Article

Enhanced photoelectrochemical water splitting with a donor-acceptor polyimide

Journal Article

Effects of mulching for water conservation on soil carbon, nitrogen and biological properties

Xiaomin PI, Tongxun ZHANG, Benhua SUN, Quanhong CUI, Yun GUO, Mingxia GAO, Hao FENG, David W.

Journal Article

Mutation analysis of KCNQ1, KCNH2, SCN5A, KCNE1 and KCNE2 genes in Chinese patients with long QT syndrome

DU Rong, TIAN Li, YUAN Guohui, LI Jin, REN Faxin, GUI Le, LI Wei, ZHANG Shouyan, KANG Cailian, YANG Junguo

Journal Article

A 3D porous WP2 nanosheets@carbon cloth flexible electrode for efficient electrocatalytic hydrogen evolution

Mingyu Pi, Xiaodeng Wang, Dingke Zhang, Shuxia Wang, Shijian Chen

Journal Article

Performance of PI controller for control of active and reactive power in DFIG operating in a grid-connected

Azzouz TAMAARAT,Abdelhamid BENAKCHA

Journal Article

Precision control system of two-DOF stage with linear ultrasonic motor

ZHANG Hanlei, SHI Yunlai, ZHAO Chunsheng

Journal Article

Development of a new method for RMR and Q classification method to optimize support system in tunneling

Asghar RAHMATI,Lohrasb FARAMARZI,Manouchehr SANEI

Journal Article

The Potential Role of Powdery Mildew-Resistance Gene <i>Pm40i> in Chinese Wheat-Breeding Programs inthe Post-<i>Pm21i> Era

Shengwen Tang,Yuting Hu,Shengfu Zhong,Peigao Luo

Journal Article